Mapping Neuronal Connectivity using Laser Photostimulation and Calcium Imaging
نویسندگان
چکیده
منابع مشابه
A portable laser photostimulation and imaging microscope.
We describe a compact microscope that uses a spatial light modulator (SLM) to control the excitation laser light. The flexibility of SLMs, which can mimic virtually any optical transfer function, enables the experimenter to create, in software, arbitrary spatio-temporal light patterns, including focusing and beam scanning, simply by calculating the appropriate phase mask. Our prototype, a scan-...
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Fluorescent calcium imaging provides a potentially powerful tool for inferring connectivity in neural circuits with up to thousands of neurons. However, a key challenge in using calcium imaging for connectivity detection is that current systems often have a temporal response and frame rate that can be orders of magnitude slower than the underlying neural spiking process. Bayesian inference meth...
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Llano DA, Theyel BB, Mallik AK, Sherman SM, Issa NP. Rapid and sensitive mapping of long-range connections in vitro using flavoprotein autofluorescence imaging combined with laser photostimulation. J Neurophysiol 101: 3325–3340, 2009. First published March 25, 2009; doi:10.1152/jn.91291.2008. We investigated the use of flavoprotein autofluorescence (FA) as a tool to map long-range neural connec...
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We investigated the use of flavoprotein autofluorescence (FA) as a tool to map long-range neural connections and combined FA with laser-uncaging of glutamate to facilitate rapid long-range mapping in vitro. Using the somatosensory thalamocortical slice, we determined that the spatial resolution of FA is >or=100-200 microm and that the sensitivity for detecting thalamocortical synaptic activity ...
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The astrocyte is a large, electrically non-excitable population in the central nervous system. Numerous evidences have proved that they actively contribute to brain functions, reciprocally communicating with neurons, and serving as a bridge between neurons and other brain cells. However, conventional stimulation approaches are hard to be feasibly utilized to activate astrocytes for delicate inv...
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ژورنال
عنوان ژورنال: Biophysical Journal
سال: 2016
ISSN: 0006-3495
DOI: 10.1016/j.bpj.2015.11.831